高校问答部落冲突暴哥布林:帮忙更改翻译。即刻给分！！！！
来源：百度文库 编辑：高校问答 时间：2024/04/29 00:23:46
摘要：本实验在EDTA和SDS等去污剂存在下，用蛋白酶K和RNA酶先后消化细胞，
随后用苯酚：氯仿：异戊醇（25：24：1）抽提，得到的DNA溶液经透析纯化后，
用蔗糖浓缩，最后用琼脂糖凝胶电泳检测。结果表明：用这种方法可以从单只
蜱中提取到纯度较高的蜱类基因组DNA。
版本2：Abstract: This experiment under detergent existences and so on the EDTA and SDS, digests the cell successively with proteinase K and the RNA enzyme,
afterwards uses the phenol: Chloroform: The isoamyl alcohol (25:24:1) extraction, obtains the DNA solution after the dialysis purification,
with the sucrose concentration, finally uses the agarose gel electrophoresis to examine. The result indicated that,May withdraw with this method from
the single tick to purity high tick class genome team DNA.
In this experiment, with the presence of detergents such as EDTA and SDS, proteinase K and RNA enzymes were used sequentially to digest the cells. That was followed by extration using a mixture of phenol, chloroform, iso-pentanol (25:24:1). The abtained DNA solution, which was purified by means of dialysis, was concentrated by use of cane sugar. Finally, it was detected and measured by gel electrophoresis. The results showed that DNA genomes with high purity can be extracted from a single tick.
In this experiment, EDTA and SDS saw there, using proteinase K digestion and RNA enzymes has cells, followed by phenol : chloroform : from e alcohol (25:24:1) extraction from the DNA sample after dialysis solution by using sugar enrichment, the final gel electrophoresis testing Qiongzhitang used. The results showed : from the use of this method can extract only Tick Tick category to the higher purity DNA genome
In this experiment, EDTA and SDS saw there, using proteinase K digestion and RNA enzymes has cells, followed by phenol : chloroform : from e alcohol (25:24:1) extraction from the DNA sample after dialysis solution by using sugar enrichment, the final gel electrophoresis testing Qiongzhitang used. The results showed : from the use of this method can extract only Tick Tick category to the higher purity DNA genome