高校问答无头东宫 迅雷下载:生物英语，可以帮忙翻译成中文吗？
来源：百度文库 编辑：高校问答 时间：2024/04/29 21:30:30
Ten iridoviruses were obtained from four different fish cultured in Korea during
epidemics from 2000 to 2002 (Fig. 1). Sources of the fish iridoviruses used in this
study are listed in Table 1.
The full-length ORF of the MCP gene was amplified from uncultured spleen DNA using polymerase chain reaction (PCR). Spleen from infected fish was
homogenized in a lysis buffer (4 M urea, 200 mM Tris, 20 mM NaCl, 200 mM EDTA, pH 7.4). After centrifugation at 1700 g for 10 min, the supernatant was
treated with proteinase K (0.2 mg/ml) and sarcosyl (1%) at 45 .C for 3 h, followed by phenol/chloroform extraction and ethyl alcohol precipitation. The genomic
DNA in the pellet was dissolved in TE (10 mM Tris-HCl and 1 mM EDTA, pH 7.5) and used as a template for PCR. Primers for PCR were designed from nucleotide
sequences in the GenBank/EMBL databases of RBIV (AY532606, Do et al. [3]). PCR primers were as follows
epidemics from 2000 to 2002 (Fig. 1). Sources of the fish iridoviruses used in this
study are listed in Table 1.
The full-length ORF of the MCP gene was amplified from uncultured spleen DNA using polymerase chain reaction (PCR). Spleen from infected fish was
homogenized in a lysis buffer (4 M urea, 200 mM Tris, 20 mM NaCl, 200 mM EDTA, pH 7.4). After centrifugation at 1700 g for 10 min, the supernatant was
treated with proteinase K (0.2 mg/ml) and sarcosyl (1%) at 45 .C for 3 h, followed by phenol/chloroform extraction and ethyl alcohol precipitation. The genomic
DNA in the pellet was dissolved in TE (10 mM Tris-HCl and 1 mM EDTA, pH 7.5) and used as a template for PCR. Primers for PCR were designed from nucleotide
sequences in the GenBank/EMBL databases of RBIV (AY532606, Do et al. [3]). PCR primers were as follows
Tris通常溶于盐酸中，我做过这个实验，试剂就叫“Tris”，翻译过来就很不好听了。
NaCl (这个不知道我打你，呵呵！)
乙二胺四乙酸（EDTA）
lysis buffer裂解缓冲液
2000到2002年从韩国四种不同的饲养鱼类中获得10种虹彩病毒 (图1)。本研究使用的鱼类虹彩病毒资源列于表1。用聚合酶链式反应 (PCR)扩增主要衣壳蛋白（MCP）基因的开放阅读框架（ORF）的总长度。被感染鱼的脾脏在裂解缓冲液中分散 (4 摩尔/升尿素，200毫摩尔/升的Tris, 20 毫摩尔/升的NaCl，毫摩尔/升乙二胺四乙酸，pH= 7.4)。1700 g 离心10分钟，上清夜用蛋白酶K (0.2 mg/ml) 和 sarcosyl (1%) 在45 .C时处理3小时，然后用苯酚/氯仿萃取再用乙醇溶解。小球中的染色体DNA溶解于“TE”液中 (10 mM Tris-盐酸和1 mM 乙二胺四乙酸，pH 7.5) 作为聚合酶链式反应（PCR）的模板。聚合酶链式反应的引物（Primers）由RBIV 的基因银行（GenBank/EMBL）数据库提供的核酸序列来设计 (AY532606, Do等[3]). PCR 引物如下………