口袋妖怪 蓝蟾蜍:小妹请大侠帮忙翻译

The fixed microorganism cell can carry on the normal growth, the reproduction and the metabolism, may be similar to using the immobilized cell drifts away the cell such fermentation to produce each kind of metabolite, therefore uses in outside each kind of butcher the product production. Moreover because fixed process simple, mechanical strength high, the anti- microorganism decomposes, precipitation separation good, the life is long and so on the merit has the widespread application prospect. Carries on fixedly take the sea alginic acid sodium as the embedding material to the acetic acid bacillus has the quite mature research □

　　In experiment to three kind of acetic acid fungi: Shanghai ferments 1 _ 1, acetic acid bacillus ZW1-3 and the acetic acid pole □_ 1 fermentation produced the acid to conduct the research, produced the sour situation to three kind of fungi to carry on the comparison. Uses the sea alginic acid sodium embedding law to carry on fixedly, has conducted the optimized research to the fixed condition, to affects the fixed quality several factors (sea alginic acid sodium density, calcium chloride density, 菌液 ratio of with the sea alginic acid sodium) carries on the orthogonal experiment, obtains the best fixed condition. And fermented to the immobilized cell and drifts away the cell fermentation to carry on the comparison □first to carry on to the mold mushroom spawn sieves selects the NaOH titrimetric method to measure the acetic acid content _ 1mol/LNaOH solution), the mycelium density with the turbidity law determination, the ultraviolet spectrophotometer, 61Onm, took produces the acid to be many, grows the quick strain, raised the strip to imitate 0 ℃, 180r/min. Selects the mold mushroom spawn does the fixed optimized experiment, fixed method flow chart following _
　　The preparation congeals in the bead process, affects the rubber bead quality the primary factor to include: The sea alginic acid sodium density, contains 菌液 the recruitment, the CaCl2 density. Therefore, does three horizontal orthogonals experiments to the above three factors. Obtains the orthogonal experiment optimum condition. First carries on the single factorial experiment maintenance calcium chloride density, the sea alginic acid sodium density, 菌液 is more invariable than with the sea alginic acid sodium the center two factors, changes one kind of density or the amount used. After the experiment observes the rubber bead the size, degree of hardness and so on obtains the quite suitable three amounts used to take three levels. Then, lists three factors three levels factors 水平表, again 水平表 lists three factors three levels orthogonal tables according to the factor, then carries on the experiment, from Cheng Qiuxing, the surface strength, degree of hardness aspect hits the minute (to see also table one). Obtains the best fixed condition □
　　Because the orthogonal experiment completely depends upon the sense organ to pinch, pinches, rubs the hands grades, therefore has a bigger error in the degree of hardness difference not big situation which grades. But has carried on the continual fermentation raise for the consolidated orthogonal result to orthogonal experiment each group, the observation fixed pellet service life □finally, the system results in the immobilized cell under the best fixed condition with to drift away the cell to carry on the single batch of fermentation to compare, according to obtains produces the sour quantity, the fermentation cycle, in the culture medium aspect the and so on mycelium density variance analysis fixed fermentation is opposite to drifts away in the merit and the shortcoming □paper which the cell ferments mainly emphatically to the fixed optimum condition determination and with drifts away the comparison which the cell ferments, its main research has following several _ I, to three kind of mold mushroom spawns which obtained arrives: The acetic acid bacillus 1.01, acetic acid bacillus ZW1-3 and Shanghai ferment 01, carries on the screening comparison, discovered acetic acid bacillus 1.01 produces the sour quantity high to grow quickly, is the ideal fermentation mold mushroom spawn □
　　IITH, to the sea alginic acid sodium, the calcium chloride and 菌液 the recruitment carries on the single factorial experiment, had determined in the experimental three factors and three levels are: Sea alginic acid sodium 2.0% _ 5% _ 0% 菌液: The sea alginic acid sodium compares 20 _ 00 _ 0 _ 00 _ 0 _ 00 CaCl2 density 1.5% _ 0% _ 5%. Carries on the orthogonal experiment to three factors three levels, had determined the fixed optimum condition is: Calcium chloride 1.5%, sea alginic acid coin 0%, 菌液: Sea alginic acid sodium □0 _ 00ml, as a result of the sense organ ability difference, has carried on the raise in the experiment to an orthogonal experiment dish type, the discovery chlorination coin 5%, sea alginic acid coin 0%, 菌液: Sea alginic acid sodium □0 _ 00ml allocated proportion in raise service life longest, the result is consistent with the sense organ judgement □
　　IIITH, the fixed optimum condition ferments and drifts away the comparison which the cell ferments to discover that, 1st, drifts away the cell to ferment the most first birth acid quickly, but the immobilized cell ferments then relative is slower. When 72 hours is fixed produced the sour quantity already to be bigger than drifts away the cell to ferment produces the sour quantity, and continued in the fermentation fluid which maintained one's supremacy or lead □2, the immobilized cell ferments the mycelium along with the single batch of fermentation time increase to increase, but was smaller than drifts away the mycelium density which the cell fermented. After this reason is the immobilized cell system is unable to clean the pellet surface the mycelium to drift away in the culture medium cell unceasingly to reproduce makes in the culture medium the mycelium to increase □the key to shift fixedly; Acetic acid bacillus; Sea alginic acid sodium; Embedding 泮